TOPIC
Gallic Acid Reversed NeuN and Mbp Reactivity in Hippocampal CA3 Doxorubicin Challenge: Enhancement of Neuronal  Integrity

AUTHOR

Okpala, Onyedikachukwu Precious, Elizabeth Finbarrs-Bello*, ,Somtochukwu Esther Onuigbo, Millicent Oluchukwu Ezennia, Vivian Onyinye Ojiakor  and Abireh Iheanacho Ezeonu

ABSTRACT
Doxorubicin is a chemotherapeutic agent that adversely affects the brain, causes deficits in learning, memory, attention, motor activity, and executive function. The impairment of cognition such as learning and memory directly affect the hippocampus. Gallic acid has antioxidant and neuroprotective activities. This study was designed to investigate the protectiveeffect of gallic acid on doxorubicin-induced neuron damage in hippocampal CA3. Thirty-two (32) albino Wistar rats were randomized into four (4) sub-groups of 8 rats each. Group 1 rats received 0.1mL of normal saline intraperitoneal for two weeks. Group 2 rats received 15mg/kg of Doxorubicin weekly intraperitoneally for two weeks.  Group 3 rats received 15mg/kg of Doxorubicin weekly intraperitoneally and orally treated with gallic acid at dose of 60mg/kg daily for two weeks. Group 4 rats received 15mg/kg of Doxorubicin weekly and orally treated with gallic acid at dose of 120mg/kg daily for two weeks. Thereafter, tissue antioxidant marker enzymes (SOD,GSH, MDA), H and E, and immunohistochemistry of NeuN and Myelin Basic Protein were carried out to ascertain the effects of Gallic acid on Doxorubicin challenge. DOX altered the antioxidant parameters by decreasing the GSH, SOD and increased MDA. The findings revealed the presence of cytoplasm vacuolations, degenerating neurons and pyknotic nuclei prominent in doxorubicin group upon haematoxylin and eosin staining, positive immunoreactivity with few neurons upon NeuN staining and decreased MBP expression. Gallic acid reversed the effects on the doxorubicin. Gallic acid dose-dependent its neuroprotective effect and would be beneficial in the doxorubicin chemotherapy when administered for longer duration.Keywords: NeuN, Myelin Basic Protein, Doxorubicin, Gallic acid, Neurotoxicity

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PUBLISHED

2023-01-11

HOW TO CITE

Okpala, Onyedikachukwu Precious, Elizabeth Finbarrs-Bello*, ,Somtochukwu Esther Onuigbo, Millicent Oluchukwu Ezennia, Vivian Onyinye Ojiakor  and Abireh Iheanacho Ezeonu (2023).Gallic Acid Reversed NeuN and Mbp Reactivity in Hippocampal CA3 Doxorubicin Challenge: Enhancement of Neuronal  Integrity. IAA Journal of Biological Sciences 10(1):68-77.